Antihyperglycemic effect of Aqueous and Ethanol extract of Aerial part of Osbeckia nepalensis Hook in Alloxan induced Diabetic rats

Aqueous and ethanolic extract of Osbeckia nepalensis Hook was evaluated for the antihyperglycemic activity in alloxan induced diabetic rats. Hyperglycemic was confirmed to Albino rats (150200 g) by treated with alloxan upto 320.6 ± 3.59 at 1 hr, 445.6 ± 4.23 at 5 days from non treated rats of 316 ± 3.56± mg/ dL. A significant hypoglycemic activity of aqueous and ethanolic extract was confirmed in alloxan induced diabetic rats (P<0.001). The serum glucose level of alloxan induced diabetic rats reduced upto 312.2 ± 3.65 and 232 ± 3.05 mg/dL at 1 hr and 120 hrs from 359±3.8 mg/ dL in aqueous extract and 320.6 ± 3.59, 445.6 ±4.24 mg /dL at 1 hr and 120 hrs. after treatment from 330 ± 3.64 mg/ dL before treatment in ethanolic extract of the test herbal. The aqueous and ethanolic extract of Osbeckia nepalensis enhance the checking of reduction in weight in comparision to normal and diabetic control animals. Further in the present work, phytochemicals of antioxidant (IC50 23 μg/ml ); saponin (20.0 ± 0.89 mg/g); flavonoid (38.28 mg/g) and minerals of K, (6.02 ± 0.51); N(22.1± 0.94); P(0.53±0.14) and Co (N.D) have screened out from the methanolic extract of Osbeckia nepalensis suggesting a unique supportive to the health care ingredients. The extracts both in aqueous and ethanolic substantiates for pharmaceutical formulation upto 4000 mg/kg oral toxicity.